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Assay Reagent Kits> KRas codon 12/13 mutations > BRaf codon 600 mutations > HBV DNA & mutationsPlasmids

KRas codon 12/13 mutations

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BRaf codon 600 mutations

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HBV DNA & mutations

 kit

 

Different Assay Formats

For each mutation or cluster of mutations, depending on the specific features of the target mutation site, several different mutation detection formats have been used to suit each researcher's needs.  These methods include:

  • mutant-specific PCR, hydrolysis (TaqMan) or non-hydrolysis probe real time PCR;
  • mutant-specific PCR combined with a PCR blocker to increase detection sensitivitity;
  • non-mutant specific PCR combined with a PCR blocker and DNA sequencing;
  • non-mutant specific PCR combined with a PCR blocker and melting curve analysis.

These methods offer different levels of sensitivity (5% to 0.01% mutant), the flexibility of detecting either one mutation at a time (mutant-specific PCR), or all potential mutations at the target site at once (sequencing or melting analysis), and being either qualitative (sequencing) or qauntitative (different format of real time PCRs).  Click here for a brief introduction of mutation detection methods.

 

Flexible Ordering

Kits can be custom assembled to fit your quantity requirement.  Price quote requests will be responded within 24-48 hours.  Academic discount available.  We accept PO numbers as well as all major credit cards.  International orders are also processed.

 












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